DNA

Part:BBa_K3595082:Design

Designed by: Xinyang Xie   Group: iGEM20_GZ_HFI   (2020-10-24)


cysteine non-sensitive L-serine O-acetyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutate several positions to diminish the inhibition of cysteine in synthesis pathway where GTC (Val), GAT (Asp) at the 95th and 96th positions are substituted for AGA (Arg), CCC (Pro); CGT (Arg), ACC (Thr) are substituted for CAT (His), GTA (Val) at 89th, 90th position; and CCG (Pro), GCA (Ala) are substituted for GCT(Ala), ACA (Thr) at 93th and 94th position.


Source

The genome of Escherichia coli.

References